Barry-Murphy sustained a second head injury in five days during last weekend`s home victory over Sheffield Wednesday.
He was replaced against the Owls after just 11 minutes by Matty Done, who had a hand in both goals in a highly impressive performance.
Hill has no other injury or suspension concerns as his men seek to further enhance their play-off credentials.
Dale lie just two points outside the play-off places but Hill warned ahead of Southampton`s visit: "We aren't safe yet and that is the number one priority.
"We have to get as much distance as we can between us and whoever is in the final relegation place."